BAMBANKER

Description

Serum-Free Cell Freezing Medium

BAMBANKER is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells and normal cells

Features

• Ready-to-use medium for preservation of cells
• Usable without diluting
• No program freezer is required
• Rapid and long-term freezing and preservation in a deep freezer (-80 C)
• Serum-free

1. Centrifuge the cells in logarithmic growth phase*, and collect 5 x 10^5 ~ 1 x 10^7 cells.
2. Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80 C without preliminary freezing. The cells frozen at -80 C can be preserved subsequently in liquid nitrogen.
3. Thawing must be carried out quickly in a constant-temperature chamber or a bath.

• Endotoxin: chromogenic substrate method
• Mycoplasma: Fluorescent antibody method
• Fungi and bacteria: As per Japanese Pharmacopoeia

Applications [Cells preserved well by cryopreservation test]:

• P3U1 (mouse myeloma cell line), K562 (human leukemia cell line), human gastric epithelial cells, etc.
• Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma), human B cell line
• OKT4 (mouse hybridoma), monkey B cell line, Activated lymphocyte derived from human peripheral blood, Activated lymphocyte derived from mouse spleen

Bambanker Publications List

1. Hikichi T., et al.: Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 25: 46-53 (2007).


2. Thuy BH, et al.: Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction, 141: 67-77 (2011).


3. Saeki K. et al.: High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells, Cloning and Stem Cells, 4: 509-22 (2009).




4. Takata Y. et al.: Generation of iPS cells using a BacMam multigene expression system. Cell Struct Funct., 36(2): 209-222 (2011)




5. Zaidi S. et al.: Runx2 deficiency and defective subnuclear targeting by pass senescence to promote immortalization and tumorigenic potential. PNAS., 104(50): 19861-19866 (2007)




6. Miono S., et al.: Characteristics and Function of Cryopreserved Bone Marrow-Derived Endothelial Progenitor Cells. Annals of Thoracic Surgery., 85: 1361-6 (2008)




7. Hatoya S., et al.: Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes, Journal of Theriogenology, 66: 1083-1090(2006)




8. Shimizu Y. et al.: Impaired Tax-specific T-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages: Cancer Sci., 100 (3): 481-9 (2009)




9. Sato D., et al.,: Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse therapy in IgA nephropathy patients., Nephrology Dialysis Transplantation, (2011)

T-Cell


10. Park HS, et al.: Bone marrow T Cells are superior to splenic T cells to induce chimeric conversion after non-myeloablative bone marrow transplantation, Korean J. Intern Med., 24 (3): 252-262 (2009)




11. Huang YH, et al.: Dental Stem Cells and Tooth Banking for Regenerative Medicine, Journal of Experimental & Clinical Medicine, 2 (3): 111-117 (2010)


12. Lee SY, et al.: Effects of Cryopreservation of Intact Teeth on the Isolated Dental Pulp Stem Cells, Journal of Endodontics, 36 (8): 1336-1340 (2010)


13. Huang MS, et al., Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp. Journal of Dental Sciences, 6(1): 48-52 (2011))


14. Agata H., et al., Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells, Differentiation, 76 (9): 981-93 (2008)




15. Kamada H., et al., In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field, American Journal of Orthodontics & Dentofacial Orthopedics, 140 (6): 799-805 (2011)




16. Mieno S., et al., Effects of diabetes mellitus on VEGF-induced proliferation response in bone marrow derived endothelial progenitor cells. J. Card Surg., 25 (5): 618-25 (2010)




17. Oshima N., et al., Optimized method for culturing outgrowth endothelial progenitor cells, inflammation and Regeneration, 31 (2): 219-227 (2011)




18. Liu DG et al, Relation between human decay-accelerating factor (hDAF) expression in pig cells and inhibition of human serum anti-pig cytotoxicity: value of highly expressed hDAF for xenotransplantation, Xenotransplantation, (1): 67-73 (2007)