B Vitamins
Isotope labelled and unlabeled vitamin reference materials for research
16 August, 2022 by
Andrew Heath

The B vitamins are a class of water-soluble organic compounds that are involved in a wide range of biochemical reactions within living organisms. The B complex includes eight chemically distinct compounds: vitamins B1 (thiamine), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxine), B7 (biotin), B9 (folate), and B12 (cobalamin).

Cambridge Isotope Laboratories, Inc. is pleased to offer a variety of stable isotope-labeled and unlabeled B vitamins and their derivatives. The standards are available in their deuterated and/or 13C/15N-labeled form in variable unit sizes for research use applications.

While the deuterated standards may be preferred due to current methods and lower costs, 13C and/or 15N standards are more desirable in practice due, for example, to isotopic stability and chromatographic alignment (to its unlabeled analogue). Please see our technical note “Benefits of 13C vs. D Standards in Clinical Mass Spectrometry Measurements” for a detailed overview of 13C (and 15N) vs. D standards in MS-based measurements.

Please search our website for the products listed below or download the brochure.

Download B Vitamin Brochure

Vitamin B Reference Materials

Vitamin B1 – Thiamine and Its Derivatives

CLM-7667 Vitamin B1 hydrochloride (thiamine hydrochloride) (4,5,4-methyl-13C3, 99%) CP 97%
DLM-8741 Vitamin B1 pyrophosphate chloride (pyrimidyl-methyl-D3, 98%)

Vitamin B2 – Riboflavin and Its Derivatives

CNLM-8851 Vitamin B2 (riboflavin) (13C4, 99%; 15N2, 98%) CP 97%
CNLM-10744 Vitamin B2 phosphate (riboflavin phosphate) (13C4, 99%; 15N2, 98%) CP 90%

Vitamin B3 – Niacin and Its Derivatives

CLM-9925 Vitamin B3 (nicotinamide) (13C6, 99%)
DLM-6883 Vitamin B3 (nicotinamide) (D4, 98%)
CNLM-9757 Vitamin B3 (nicotinamide) (2,6,carbonyl-13C3, 99%; ring-1-15N, 98%)
CLM-9954 Vitamin B3 (nicotinic acid) (13C6, 99%)
DLM-4578 Vitamin B3 (nicotinic acid) (D4, 98%)
CNLM-9512 Vitamin B3 (nicotinic acid) (2,6,carboxyl-13C3, 99%; 15N, 98%) CP 97%

Vitamin B5 – Pantothenic Acid

CNLM-7694 Vitamin B5, calcium salt·H2O (calcium panthothenate·H2O) (beta-alanyl-13C3, 99%; 15N, 98%)

Vitamin B6 – Pyridoxal 5’-Phosphate and Its Derivatives

DLM-9069 Vitamin B6 (pyridoxal) (methyl-D3, 98%)
DLM-9119 Vitamin B6 (pyridoxamine·2HCl) (methyl-D3, 98%)
CLM-7563 Vitamin B6 (pyridoxine·HCl) (4,5-bis(hydroxymethyl)-13C4, 99%)
DLM-8754 Vitamin B6 (pyridoxine·HCl) (5-hydroxymethyl-D2, 98%)
DLM-9121 Vitamin B6 (pyridoxine·HCl) (methyl-D3, 98%) CP 96%
DLM-9793 Vitamin B6 phosphate (pyridoxal phosphate) (methyl-D3, 97%) (mix of 5-,3-isomers) CP 97%

Vitamin B7 – Biotin

DLM-8806 Vitamin B7 (biotin) (ring-6,6-D2, 98%) CP 97%
DLM-9751 Vitamin B7 (biotin) (3’,3’,4’,4’-D4, 98%) CP 95%

Vitamin B9 – Folate and Its Derivatives

CLM-9548 5-Methyltetrahydrofolic acid (glutamic acid-13C5, 99%) CP 95%
CLM-7321-N 5-Methyltetrahydrofolic acid, calcium salt (glutamic acid-13C5, 98%) CP 95%
CLM-7861 Vitamin B9 (folic acid) (glutamic acid-13C5, 95%) contains ~10% H2O
CLM-7861-N Vitamin B9 (folic acid) (glutamic acid-13C5, 99%) CP 95%

Vitamin B12 – Cobalamin

CLM-9770-E Vitamin B12 (cyanocobalamin) (13C7, 99%) CP 95%

Research References

Huang, J.; Cui, L.; Natarajan, M.; et al. 2022. The ratio of nicotinic acid to nicotinamide as a microbial biomarker for assessing cell therapy product sterility. Mol Ther Methods Clin Dev, 25, 410-424.

Marshall, J.; Zhang, H.; Khazaei, H.; et al. 2021. Targeted quantification of B vitamins using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry in faba bean seeds. J Food Compost Anal, 5, 103687-103697.

Zhang, H.: De Silva, D.; Dissanayaka, D.; et al. 2021. Validated B vitamin quantification from lentils by selected reaction monitoring mass spectrometry. Food Chem, 359, 129810-12918.

Shetty, S.A.; Young, M.F.; Taneja, S.; et al. 2020. Quantification of B-vitamins from different fresh milk samples using ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring methods. J Chromatogr A,1609, 460452-460487.

McClure S. 2020. Simultaneous determination of total vitamins B1, B2, B3, and B6 in infant formula and related nutritionals by enzymatic digestion and LC-MS/MS – A multi- laboratory testing study final action: AOAC Method 2015.14. J AOAC Int, 103(4), 1060-1072.

Gill, B.D.; Saldo, S.; Wood, J.E.; et al. 2018. A rapid method for the determination of biotin and folic acid in liquid milk, milk powders, infant formula, and milk-based nutritional products by liquid chromatography-tandem mass spectrometry. J AOAC Int, 101(5), 1578-1583.

Paalme, T.; Vilbaste, A.; Kevvai, K.; et al. 2017. Assessment of bioavailable B vitamin content in food using in vitro digestibility assay and LC-MS SIDA. Anal Bioanal Chem, 409(27), 6475-6484.