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Over the years, stable isotopes,
NMR and mass spectrometry have emerged as important tools for metabolic
research, nutritional studies, molecular biology, quantitative proteomics,
drug discovery and protein structural analysis. These non-radioactive
isotopes are well documented in the scientific literature as safe
and effective probes for the in vivo investigation of biochemical
pathways.
Cambridge Isotope Laboratories Inc. (CIL) has developed a comprehensive inventory of products used in metabolic, protein, nucleic acid or other biochemical research. These include labelled carbohydrates, amino acids, fatty acids, triglycerides and their biosynthetic precursors or metabolites (many of which are available as metabolic grade or sterility and pyrogenicity tested material), cell growth media, uniformally and specifically labelled nucleosides and nucleotides, protein NMR standards and buffers. CIL also offers custom protein expression and custom media formulation.
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The Protein NMR range of products includes:
RECOMBINANT PROTEIN EXPRESSION PRODUCTS
Minimal Media Nutrients
● Ammonium Chloride (15N)
● Ammonium Sulfate (15N)
● D-Glucose (U-13C6)
● D-Glucose (1,2,3,4,5,6,6-D7)
● D-Glucose (U-13C6; 1,2,3,4,5,6,6-D7)
● Deuterium Oxide (99%, 99.8% or 99.9%)
BioExpress 1000 - a fully-rich medium made from
algal hydrolysate for bacterial expression systems, containing amino
acids, peptides, nucleic acids, sugars, salts, vitamins and a variety
of macronutrients. No spiking is necessary. Bio-Express-1000 may
itself be used as a supplement to minimal media for economical production
of labelled protein.
Celtone Base Powder - a mixture of amino acids,
peptides, vitamins and other essential nutrients, which provide a "rich"
environment for excellent bacterial cell growth and high protein
expression. The advantage of Celtone powder is that researchers can
formulate a custom medium based on their specific research needs.
This product alone does not constitute a complete medium - appropriately
labelled salts and carbohydrates are needed to make a complete medium.
Celtone Complete Medium - a "rich" bacterial cell growth
medium derived from an algal source with a growth rate comparable to LB,
allowing for inoculation and induction within one working day. Celtone
contains amino acids, nucleic acids, peptides, vitamins, salts and other
essential nutrients, and provides excellent cell growth and high protein
expression. It is formulated as ready-to-use medium without need for
dilution or pH adjustment.
Spectra 9 - a const-effective medium for E. coli
bacterial growth and protein expression. It is comprised of labelled
salts and labelled carbohydrates, and is supplemented with Celtone Base
Powder (1g powder per 1L Spectra 9) which contains amino acids, vitamins,
peptides and other essential nutrients. Spectra 9 is available as a
ready-to-use solution and should not be diluted.
BioExpress 2000 - a fully-rich, serum-free medium
for insect cell growth and the Baculovirus Expression Vector System
(BEVS). It is packaged as two components: a solid powder (a proprietary
blend of inorganic salts and labelled amino acids) and a liquid
component (fatty acid soluition). Amino acid selective labelling is
possible with BioExpress 2000 because the amino acid content is
chemically defined.
BioExpress 6000 - this media should work well with cell
lines grown in DMEM. Amino acid selective labelling is possible with
BioExpress 6000 because the amino acid content is chemically defined.
Labelled Amino Acids - a comprehensive range of
amino acids labelled with 15N, 13C and D.
a-Keto Acids - Various labelled varieties of :
● a-Ketobutyric Acid, Sodium Salt
● a-Ketoisocaproic Acid, Sodium Salt
● a-Ketoisovaleric Acid, Sodium Salt
● Sodium Pyruvate
CELL FREE PROTEIN SYNTHESIS
● Algal Amino Acid Mix (contains 16 amino acids)
● "Cell Free" Amino Acid Mix (contains 20 amino
acids)
● Qiagen Kits (for large-scale production by in vitro translation)
PEPTIDE SYNTHESIS PRODUCTS
CIL offer a comprehensive range of isotopically labelled protected
amino acids (N-FMOC and N-t-BOC derivatives).
RNA/DNA PRODUCTS
CIL offer a range of labelled nucleosides, nucleotide monophosphates,
nucleotide triphosphates and phosphoramidites.
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Please contact
us for prices or to ask for the current CIL BioNMR catalogue.
Alternatively, you may search CIL's
website for more information about these products.
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The Metabolic range of products includes:
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For Glucose Metabolism & Recycling:
● L-Alanine (U-13C3)
● D-Glucose (U-13C6)
● D-Glucose (2-D)
● D-Glucose (6,6-D2)
● Phosphoenol pyruvate (2,3-13C2)
● Sodium Acetate (2-13C)
● Sodium L-Lactate (13C3)
For Amino Acid Kinetics:
● a-Ketoisocaproic Acid (1-13C)
● L-Leucine (5,5,5-D3)
● L-Leucine (15N)
● L-Leucine (U-13C6; 15N)
For Protein Metabolism:
● Glycine (15N)
● L-Leucine (1-13C)
● a-Ketoisocaproic Acid (1-13C)
● L-Phenylalanine (ring-D5)
● L-Tyrosine (3,3-D2)
● L-Tyrosine (ring-D4
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For DNA Systhesis Rates:
● Glucose (6,6-D2)
● Glycine (1-13C)
For Lipolysis Rates and Reesterification:
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Glycerol (1,1,2,3,3-D5)
● Glycerol (1,2,3-13C3)
● Palmitic Acid (1-13C)
● Sodium Palmitate (1-13C)
For Urea Metabolism:
● L-Arginine.HCl (guanido-15N2)
● L-Ornithine.HCl (3,3,4,4,5,5-D6)
● Urea (15N2)
For Energy Expenditure and Body Composition:
● Water (18O, 10%)
● Water (18O, 95%+)
● Deuterium Oxide
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Please contact us for prices or to ask for the current CIL Stable Isotope Products for Metabolic Research catalogue. Alternatively, you may search CIL's website for more information about these products, or click here to download a copy.
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