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Over the years, stable isotopes and mass spectrometry have emerged as important tools for metabolic research, nutritional studies, molecular biology, proteomics, drug discovery and protein structural analysis. These non-radioactive isotopes are well documented in the scientific literature as safe and effective probes for the in vivo investigation of biochemical pathways.
Cambridge Isotope Laboratories Inc. (CIL) has developed a comprehensive inventory of products used in metabolic, protein, nucleic acid or other biochemical research. These include labelled carbohydrates, amino acids, fatty acids, triglycerides and their biosynthetic precursors or metabolites (many of which are available as metabolic grade or sterility and pyrogenicity tested material), cell growth media, uniformally and specifically labelled nucleosides and nucleotides, protein NMR standards and buffers. CIL also offers custom protein expression and custom media formulation.
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For Glucose Metabolism & Recycling:
● L-Alanine (U-13C3)
● D-Glucose (U-13C6)
● D-Glucose (2-D)
● D-Glucose (6,6-D2)
● Phosphoenol pyruvate (2,3-13C2)
● Sodium Acetate (2-13C)
● Sodium L-Lactate (13C3)
For Amino Acid Kinetics:
● a-Ketoisocaproic Acid (1-13C)
● L-Leucine (5,5,5-D3)
● L-Leucine (15N)
● L-Leucine (U-13C6; 15N)
For Protein Metabolism:
● Glycine (15N)
● L-Leucine (1-13C)
● a-Ketoisocaproic Acid (1-13C)
● L-Phenylalanine (ring-D5)
● L-Tyrosine (3,3-D2)
● L-Tyrosine (ring-D4
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For DNA Systhesis Rates:
● Glucose (6,6-D2)
● Glycine (1-13C)
For Lipolysis Rates and Reesterification:
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Glycerol (1,1,2,3,3-D5)
● Glycerol (1,2,3-13C3)
● Palmitic Acid (1-13C)
● Sodium Palmitate (1-13C)
For Urea Metabolism:
● L-Arginine.HCl (guanido-15N2)
● L-Ornithine.HCl (3,3,4,4,5,5-D6)
● Urea (15N2)
For Energy Expenditure and Body Composition:
● Water (18O, 10%)
● Water (18O, 95%+)
● Deuterium Oxide
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Please contact us for prices or to ask for the current CIL Stable Isotope Products for Metabolic Research catalogue. Alternatively, you may search CIL's website for more information about these products, or click here to download a copy.
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The Protein NMR range of products includes:
RECOMBINANT PROTEIN EXPRESSION PRODUCTS
Minimal Media Nutrients
● Ammonium Chloride (15N)
● Ammonium Sulfate (15N)
● D-Glucose (U-13C6)
● D-Glucose (1,2,3,4,5,6,6-D7)
● D-Glucose (U-13C6; 1,2,3,4,5,6,6-D7)
BioExpress 1000 - a fully-rich medium made from algal hydrolysate for bacterial expression systems, containing amino acids, peptides, nucleic acids, sugars, salts, vitamins and a variety of macronutrients. No spiking is necessary. Bio-Express-1000 may itself be used as a supplement to minimal media for economical production of labelled protein.
Silantes E.Coli-OD2 - a medium made from bacterial hydrolysate, containing primarily amino acids, low molecular weight oligopeptidesand almost no carbohydrates. The bacterial strain used is a chemolithoautotrophic organism which grows on inorganic substrates that are isotopically labelled.
BioExpress-Min - a fortified minimal medium for economical expression in bacterial systems. Bio-Express-Min delivers more than double the growth seen in traditional minimal media, and usually no further augmentation of the media is necessary.
BioExpress 2000 - a fully-rich, serum-free medium for insect cell growth and the Baculovirus Expression Vector System (BEVS).
BioExpress YBN - a fully-rich medium derived from yeast biomass for high expression yields of both prokaryotic and eukaryotic proteins in E. coli and yeast.
Silantes Yeast-OD2 - a medium made from bacterial hydrolysate for the expression of eukaryotic proteins.
BioExpress 6000 - currently under development, this medium will be ideal for mammalian cell culture.
MonoExpress 1500 - A fully-rich isotope-depleted medium, made from a yeast cell hydrolysate.
Labelled Amino Acids - a comprehensive range of amino acids labelled with 15N, 13C and D.
a-Keto Acids - Various labelled varieties of :
● a-Ketobutyric Acid, Sodium Salt
● a-Ketoisocaproic Acid, Sodium Salt
● a-Ketoisovaleric Acid, Sodium Salt
● Sodium Pyruvate
CELL FREE PROTEIN SYNTHESIS
● Algal Amino Acid Mix (contains 16 amino acids)
● "Cell Free" Amino Acid Mix (contains 20 amino acids)
PEPTIDE SYNTHESIS PRODUCTS
CIL offer a comprehensive range of isotopically labelled protected amino acids (N-FMOC and N-t-BOC derivatives).
RNA/DNA PRODUCTS
CIL offer a range of labelled nucleosides, nucleotide monophosphates, nucleotide triphosphates and phosphoramidites. |
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Please contact us for prices or to ask for the current CIL Structural Genomics and Proteomics catalogue. Alternatively, you may search CIL's website for more information about these products.
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